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Department

Immunology
TSPOT

Preferred Sample Type

T-SPOT.TB ( gamma interferon TB test)

Suitable Specimen Types

  • Li Hep
• Adults and children (> 10 years): one 6 mL LiHep tube. • Children 2 - 9 years: one 4 mL LiHep tube. • Children < 2 years: one 2 mL LiHep paediatric tube. Blood should be stored at room temperature (no refrigeration) and assayed within eight hours.

Sample Processing in Laboratory

To arrive in laboratory by 2.00 pm. No Friday service. T-SPOT assay requires cell culture overnight. Blood should be collected and ideally progressed within eight hours of collection. This time can be prolonged by using the T-cell Xtend reagent.

Turnaround Time

7 Days

T-SPOT.TB ( gamma interferon TB test)

General Information

T-Spot test can be used to detect exposure to Mycobacterium Tuberculosis. This assay is currently not available through Primary Care

Patient Preparation

none

Notes

Assay run Monday through thursday.Please phone laboratory (42184) to arrange.

Reference Range

RESULTS - Positive, Negative, Borderline and Indeterminate results. 

A negative result suggests no active immune response to Mycobacterium Tuberculosis.  A positive results indicates a response to MTB infection, but does not distinguish between active and latent disease. The test cannot be used to exclude active infection, since false-negative results do occur. It should always be interpreted in the context of other clinial and laboratory features.

Due to biological variations, re-testing of borderline (equivocal) results is recommended. Indeterminate results indicate failure of positive or negative controls which may be due to technical or patient-specific factors.  Re-testing recommended.  

Specifications

  • EQA Scheme?: Yes