Protocol for Antibiotic Assays

Samples For all antibiotic assays, 5-10mls blood into red-topped tube (no anticoagulant) DO NOT sample from the line through which the antibiotic has been given.

 

Antibiotic Sampling Time First Sample Expected Levels Usual interval to re-assay

 

VANCOMYCIN

 

Pre-dose only (i.e. within 15 mins before dose)

 

Around 2nd – 4th dose

10 – 20mg/L

3 days

 

GENTAMICIN / TOBRAMYCIN (b.d or t.d.s regime)

Pre-dose (within 15 mins before dose)

and

Post-dose (1hr after dose)

Around 2nd – 4th dose

Pre less than 2mg/L

Post 5 – 10 mg/L

3 days

GENTAMICIN / TOBRAMYCIN (once – daily regime) Post-dose only taken between 6 and 14 hours after dose.

N.B. Times of dosing and sampling must be given on request form.

1st to 3rd dose

Interpretation by Microbiologist

3 days

Important Notes

  1. After taking pre-dose levels, give the prescribed dose as normal – DO NOT wait for the results of the assay.
  2. “Random” levels should seldom be needed and should only be taken by prior arrangement with the Duty Microbiologist.
  3. For patients with stable renal function, whose results are within the expected range, assays do not need to be performed more than twice a week. Where renal function is unstable or poor, assays should be discussed with the Duty Microbiologist.
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The laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link: https://www.ukas.com/find-an-organisation/

  • Heartlands, Good Hope and Solihull Hospital pathology laboratories are a UKAS accredited medical laboratory No.8217
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database: http://www.heftpathology.com/frontpage/test-database.html.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235

UKAS HEFT