Preferred Sample Type

Alkaline Phosphatase (ALP)

Suitable Specimen Types

  • Serum
  • Li Hep Plasma
200 uL blood (1 x paed Lith Hep tube)

Specimen Transport

Not applicable

Sample Processing in Laboratory

No special requirements

Sample Preparation

Centrifuge on arrival

Turnaround Time

1 day

Sample Stability


Alkaline Phosphatase (ALP)

General Information

ALP activity changes markedly throughout childhood and rises rapidly during the adolescent growth spurt, particularly in boys. Increases in bone ALP of 3.4-4.0 times the adult range can be seen during this time. Values of over 1000 U/L in teenage boys or 800 U/L in teenage girls require investigation.

Patient Preparation



No significant interference from haemolysis, icterus or lipaemia.

EDTA contamination decreases ALP activity.

Anticonvulsants, barbiturates, oral contraceptives can increase ALP activity.

Reference Range

Neonate (<4weeks)   70-380 U/L

4wks-16yrs               60-425 U/L

Adult                        30-130 U/L

(Source : Pathology Harmony Recommendations)


  • EQA Scheme?: Yes
  • EQA Status:

    UK NEQAS for Clinical Chemistry

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The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235