Non-haemolysed serum or heparinised or EDTA plasma from fasting patients are the specimens of choice. Both direct and total bilirubin undergo photo-oxidation or dehydrogenation to biliverdin or other intermediate products upon exposure to white light, either artificial or sunlight.
Serum bilirubin may drop as much as 50% after one hour exposure to sunlight. For optimal stability, both darkness and low temperature are necessary.
At room temperature, in total darkness, samples are stable for 2 days. Samples are stable for 4 to 7 days in the refrigerator and frozen samples are stable for 3 months if totally protected from light.
Bilirubin measurements are used in the diagnosis and monitoring of liver disease, haemolytic anaemia and in the assessment of severe jaundice, especially in neonates.
Lipaemia and haemolysis cause interference in samples from adults. Haemolysis does not cause interference in neonatal samples.
14 days - Adult <21 umol/L
(Source : Pathology Harmony Recommendations)
WEQAS and NEQAS
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Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.
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