Preferred Sample Type

Female Testosterone

Suitable Specimen Types

  • Serum
1 mL

Sample Preparation

DO NOT separate serum into Impeco tubes as this causes interference with the assay.

Turnaround Time

10 days

Sample Stability

A 1mL serum sample is required. Separated serum samples stored in plastic tubes 4°C for up to 7 days can be used for analysis. Additionally, primary gel tubes can be used for analysis, female testosterone is stable for up to 7 days when the primary tube is stored at 4°C.

Female Testosterone

General Information

When and Why to get tested:


If you are a female but have male traits, such as a low voice or excessive body hair (hirsutism), or are infertile or have no periods or if they are irregular


- Infertility issues


- premature or delayed puberty


- masculine physical features


-To investigate polycystic ovarian syndrome (PCOS)













Patient Preparation



In females, testosterone is analysed using a specific tandem mass spectrometry method, as the usual immunoassay method is prone to interferences in females.

Reference Range

Female Testosterone < 1.6nmol/L (derived from 17 healthy females)

Levels >4nmol/L should be referred to gynaecology for further investigations e.g. ultrasound scan


  • EQA Scheme?: Yes
  • EQA Status: NEQAS

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HTA licence number is 12366

Protection of Personal Information – Laboratory Medicine comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235