Preferred Sample Type

H.pylori Stool Antigen

Suitable Specimen Types

  • Faeces
2- 3 g or >5mls

Specimen Transport

Samples must arrive in the laboratory within 48 hours of collection

Turnaround Time

4 days  

H.pylori Stool Antigen

General Information

This test is used to diagnose infection due to Helicobacter pylori.

A positive H. pylori test indicates that your gastrointestinal pain may be due to a peptic ulcer caused by this bacteria. Antibiotic treatment will erradicate the  H.pylori infection and the associated symptoms will be alleviated.

The H.pylori Stool antigen is reliable both in the diagnostic and post eradication settings provided the patient has avoided PPI’s for 2 weeks and antibiotics for 4 weeks prior to sample collection as they may interfere with this assay.

The Urea breath test was always the gold standard but a lack of standardisation and quality control means it is not always a reliable test.

Reference Management of Helicobacter pylori infection the Maastricht IV/ Florence Consensus Report. Peter Malfertheiner et al. The European Helicobacter Study Group (EHSG)

Patient Preparation

Samples must arrive in the laboratory within 48 hours of collection. Patients should be advised to bring the stool sample to the GP surgery or laboratory reception as soon as possible after collection, ideally same or next day.

Please note 2-3g/5ml must be provided otherwise the sample will be rejected. Any unlabelled, mismatched, leaking or incorrect sample will be rejected.

Reference Range

Qualitative test.  


  • EQA Scheme?: Yes

The laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link:

  • Heartlands, Good Hope and Solihull Hospital pathology laboratories are a UKAS accredited medical laboratory No.8217
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database:

Protection of personal information - Laboratory Medicine comply with the Trust Data Protection policy and have procedures in place to allow the Directorate and its employees to comply with the Data Protection act  1998 and associated best practice and guidance.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235