Genotypic HBV resistance testing is the current recommended method for determining drug resistance in HBV infection. Genotypic resistance data provides information on the presence/absence of drug resistance mutations, which is used as a predictor of virological response to therapy. This aids clinical decisions regarding therapy changes. Choosing the most appropriate therapy regime improves patient management and reduces the cost of care.
The methodology for genotypic HBV resistance testing involves extraction of DNA, amplification of the polymerase gene using a nested PCR, population sequencing, sequence analysis and resistance interpretation.
For taking blood samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of Clinical Nursing Procedures”.
Lavender top blood (EDTA) sample
The Hepatitis B genotypic resistance request form is available from the following link HBV GENOTYPIC RESISTANCE REQUEST FORM
The laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link: https://www.ukas.com/find-an-organisation/
Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.
For further test information, please visit the test database: http://www.heftpathology.com/frontpage/test-database.html.
Protection of personal information - Laboratory Medicine comply with the Trust Data Protection policy and have procedures in place to allow the Directorate and its employees to comply with the Data Protection act 1998 and associated best practice and guidance.
For further information contact Louise Fallon, Quality Manager, 0121 424 1235